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KMID : 0644120060190010166
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Journal of Oriental Gynecology
2006 Volume.19 No. 1 p.166 ~ p.177
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Anti-proliferative effect of Sam-nueng(Sparganii Rhizoma) extract on MCF-7 cells
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Park Kyung-Mi
Jo Sung-Hee
Jeong Kyoung-Ah
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Abstract
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Purpose : This investigation was undertaken to evaluate the antiproliferation, atoptosis of Sam-nueng(Sparganii Rhizoma) extract using MCF-7 human breast cancer cells.
Methods : MCF-7 cells were cultured in Dulbecco’’s modified Eagle’’s medium/F12(DMEM/F12) supplemented with 10 % fetal bovine serum(FBS; Gibco) and antibiotics. At varying times after extract treatment, cells were harvested with scraper and processed for analysis of protein expression, proliferation, cytotoxicity and apoptosis.
Results : Our results show that the extract of Sam-nueng(Sparganii Rhizoma) strongly inhibits the proliferation of MCF-7 cells in a dose and time-dependent manner. Sulforhodamine B showed that the addition of Sam-nueng(Sparganii Rhizoma) extract reduced the viability of MCF-7 cells in a dose-dependent manner. Poly[ADP(ribose)] polymerase(PARP) which serves as a marker of cells undergoing apoptosis, a major substrate for caspase-3 was extensively cleaved in the Sam-nueng(Sparganii Rhizoma)-treated cells.
Conclusion : So, we can conclude that Sam-nueng(Sparganii Rhizoma) can have an inhibitive effect on MCF-7 human breast cancer cells by variable mechanisms.
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KEYWORD
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human breast cancer, MCF-7, Sam-nueng(Sparganii Rhizoma), antiproliferation, cytotoxicity, apoptosis
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